CHST4 associates with high-abundance immune infiltration in hormone receptor–positive breast cancer
Hormone receptor-positive breast cancer (HR+ BRCA) with high-risk factors such as lymph node metastasis has a relatively poor prognosis. However, the biological basis of tumor cell migration is still poorly understood, especially as some of the metastatic events occur at an early stage. Here, we identified that CHST4 (carbohydrate sulfotransferase 4), which has an important role in lymphocyte homing, was abnormally downregulated in HR+ BRCA and associated with lymph node metastasis. By enrichment analysis and immune infiltration evaluation, we predicted the potential ability of CHST4 to enhance immune cell infiltration. Then, immunohistochemical staining further demonstrated the contribution of CHST4 to the infiltration abundance of CD8+ T cells and CD4+ T cells in HR+ BRCA. Immunohistochemical staining of MECA-79 further identified the correlation between CHST4 and sulfated peripheral node addressin. Finally, we demonstrated that CHST4 was connected to increased tumor-immune cell communication by analyzing single-cell sequencing data. In summary, our study provided novel insights into the regulation of HR+ BRCA immune infiltration by CHST4.
Authors
- Anbang Hu (ORCID: https://orcid.org/0000-0003-0432-8081)
- Jianyuan Feng (ORCID: https://orcid.org/0000-0001-5724-4338)
- Tianshui Yu (ORCID: https://orcid.org/0000-0003-4926-3197)
- Yuhang Shang
- Mingcui Li (ORCID: https://orcid.org/0000-0002-9413-7723)
- Zhiyuan Rong
- Yunqiang Duan (ORCID: https://orcid.org/0000-0003-0143-9368)
- Yu Ding (ORCID: https://orcid.org/0000-0003-3497-6979)
- Weilun Cheng (ORCID: https://orcid.org/0000-0002-7905-7303)
- Yanling Li (ORCID: https://orcid.org/0000-0002-7764-3819)
- Zhengbo Fang
- Jiarui Zhang (ORCID: https://orcid.org/0000-0003-4776-852X)
- Jiangwei Liu (ORCID: https://orcid.org/0000-0002-7652-9379)
- Hanyu Zhang (ORCID: https://orcid.org/0000-0001-9942-8186)
- Baoliang Guo (ORCID: https://orcid.org/0000-0002-4022-6739)
- Fanjing Kong (ORCID: https://orcid.org/0000-0003-4305-8264)
- Yansong Liu (ORCID: https://orcid.org/0000-0003-1858-5468)
- S. Shakila
- Ting Wang (ORCID: https://orcid.org/0000-0003-1486-9156)
Institutions
- Harbin Medical University (CN)
- Daqing Oilfield General Hospital (CN)
- Second Affiliated Hospital of Harbin Medical University (CN)
Publication Details
- Journal
- Journal of Leukocyte Biology
- Published
- 2024-08-30
- DOI
- https://doi.org/10.1093/jleuko/qiae190
- Citations
- 1
- Primary Topic
- Immunotherapy and Immune Responses
- Type
- article
- Field-Weighted Citation Impact
- 0.24
Funders
- National Natural Science Foundation of China