Conjugal plasmid transfer in the plant rhizosphere in the One Health context
<i>K. variicola</i> EEF15 was able to colonize the lettuce rhizosphere and inhabit its leaf endosphere 7 days past bacterial administration. Fluorescence stereomicroscopy revealed plasmid transfer at a frequency of 10<sup>-3</sup>; cell sorting allowed the selection of the transconjugants. The conjugation rates and the strain's ability to colonize the plant rhizosphere and leaf endosphere make strain EEF15::<i>lacI<sup>q</sup>-pLpp-mCherry-gm<sup>R</sup></i> with pKJK5::Plac::<i>gfp</i> an interesting candidate to study ARG spread in the agri-food ecosystem. Future studies taking advantage of additional environmental donor strains could provide a comprehensive snapshot of AMR spread in the One Health context.
Authors
- Sara Borin (ORCID: https://orcid.org/0000-0001-7479-8199)
- Ester M. Eckert (ORCID: https://orcid.org/0000-0002-7314-1715)
- Barth F. Smets (ORCID: https://orcid.org/0000-0003-4119-6292)
- Arnaud Dechesne (ORCID: https://orcid.org/0000-0002-6638-2158)
- Francesca Mapelli (ORCID: https://orcid.org/0000-0002-2928-6538)
- Valentina Riva (ORCID: https://orcid.org/0000-0002-4915-4874)
- Elena Crotti (ORCID: https://orcid.org/0000-0002-3466-8274)
- Francesco Riva (ORCID: https://orcid.org/0000-0001-6739-2633)
Institutions
- University of Milan (IT)
- Aarhus University (DK)
- Water Research Institute (IT)
- Technical University of Denmark (DK)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2024-08-29
- DOI
- https://doi.org/10.3389/fmicb.2024.1457854
- Citations
- 4
- Primary Topic
- Composting and Vermicomposting Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.33