Microplastic removal and risk assessment framework in a constructed wetland for the treatment of combined sewer overflows

Combined sewer overflows (CSOs) release a significant amount of pollutants, including microplastics (MPs), due to the discharge of untreated water into receiving water bodies. Constructed Wetlands (CWs) offer a promising strategy for CSO treatment and have recently attracted attention as a potential solution for MP mitigation. Nevertheless, limited research on MP dynamics within CSO events and MP removal performance in full-scale CW systems poses a barrier to this frontier of application. This research aims to address both these knowledge gaps, representing the first investigation of a multi-stage CSO-CW for MP removal. The study presents one year of seasonal data from the CSO-CW upstream of the WWTP in Carimate (Italy), evaluating the correlation of MP abundance with different water quality/quantity parameters and associated ecological risks. The results show a clear trend in MP abundance, which increases with rainfall intensity. The strong correlation between MP concentration, flow rate, and total suspended solids (TSS) validates the first flush phenomenon hypothesis and its impact on MP release during CSOs. Chemical characterization identifies acrylonitrile-butadiene-styrene (ABS), polyethylene (PE), and polypropylene (PP) as predominant polymers. The first vertical subsurface flow (VF) stage showed removal rates ranging from 40 % to 77 %. However, the unexpected increase in MP concentrations after the second free water surface (FWS) stage suggests the stochasticity of CSO events and the different hydraulic characteristics of the CW units have diverse effects on MP retention. These data confirm filtration as the main retention mechanism for MP within CW systems. The MP ecological risk assessment indicates a high-risk category for most of the water samples, mainly related to the frequent presence of ABS fragments. The results contribute to the current understanding of MPs released by CSOs and provide insights into the performance of different treatment units within a large-scale CSO-CW system, suggesting the requirement for further attention.