Isolation and characterization of two novel bacteriophages against carbapenem-resistant Klebsiella pneumoniae
The increase of antibiotic-resistant bacteria has become a global health emergency and the need to explore alternative therapeutic options arises. Phage therapy uses bacteriophages to target specific bacterial strains. Phages are highly specific and can target resistant bacteria. Currently, research in this regard is focused on ensuring reliability and safety to bring this tool into clinical practice. The first step is to conduct comprehensive preclinical research. In this work, we present two novel bacteriophages vB_Kpn_F13 and vB_Kpn_F14 isolated against clinical carbapenem-resistant <i>Klebsiella pneumoniae</i> strains obtained from hospital sewage. Multiple studies <i>in vitro</i> were conducted, such as sequencing, electron microscopy, stability, host range infectivity, planktonic effect and biofilm inhibition in order to discover their ability to be used against carbapenem-resistant <i>K. pneumoniae</i> pathogens causing difficult-to-treat infections.
Authors
- Mateo Seoane‐Blanco (ORCID: https://orcid.org/0000-0003-4704-3131)
- Meritxell García‐Quintanilla (ORCID: https://orcid.org/0000-0003-1889-1377)
- Mark J. van Raaij (ORCID: https://orcid.org/0000-0002-4781-1375)
- Abrar Senhaji-Kacha (ORCID: https://orcid.org/0000-0003-0031-0976)
- John Jairo Aguilera-Correa (ORCID: https://orcid.org/0000-0002-4002-1944)
- Mireia Bernabéu-Gimeno (ORCID: https://orcid.org/0000-0001-9141-7482)
- Pilar Domingo‐Calap (ORCID: https://orcid.org/0000-0003-2829-8809)
- Jaime Esteban (ORCID: https://orcid.org/0000-0002-8971-3167)
- Sara Otaegi-Ugartemendia
Institutions
- Consejo Superior de Investigaciones Científicas (ES)
- Universitat de València (ES)
- Centro Nacional de Biotecnología (ES)
- Centro de Investigación Biomédica en Red (ES)
- Hospital Universitario Fundación Jiménez Díaz (ES)
- Institute for Integrative Systems Biology (ES)
- Universidad Autónoma de Madrid (ES)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2024-08-29
- DOI
- https://doi.org/10.3389/fcimb.2024.1421724
- Citations
- 6
- Primary Topic
- Bacteriophages and microbial interactions
- Type
- article
- Field-Weighted Citation Impact
- 2.75
Funders
- Ministerio de Ciencia, Innovación y Universidades
- European Commission
- Generalitat Valenciana
- Instituto de Salud Carlos III
- Agencia Estatal de Investigación
- European Social Fund