Integration of mass cytometry and mass spectrometry imaging for spatially resolved single-cell metabolic profiling
The integration of spatial omics technologies can provide important insights into the biology of tissues. Here we combined mass spectrometry imaging-based metabolomics and imaging mass cytometry-based immunophenotyping on a single tissue section to reveal metabolic heterogeneity at single-cell resolution within tissues and its association with specific cell populations such as cancer cells or immune cells. This approach has the potential to greatly increase our understanding of tissue-level interplay between metabolic processes and their cellular components. The authors present a workflow integrating imaging mass cytometry and imaging mass spectrometry to deconvolute metabolic heterogeneity at the single-cell level.
Authors
- Joana B. Nunes (ORCID: https://orcid.org/0000-0002-8968-9670)
- Marieke E. Ijsselsteijn (ORCID: https://orcid.org/0000-0002-8195-736X)
- Manon van der Ploeg (ORCID: https://orcid.org/0000-0001-7150-3068)
- Ahmed Mahfouz (ORCID: https://orcid.org/0000-0001-8601-2149)
- Martin Giera (ORCID: https://orcid.org/0000-0003-1684-1894)
- Tamim Abdelaal (ORCID: https://orcid.org/0000-0003-2719-5736)
- Bram Heijs (ORCID: https://orcid.org/0000-0001-6328-9305)
- Rick Ursem (ORCID: https://orcid.org/0000-0002-1607-8457)
- Noel F.C.C. de Miranda (ORCID: https://orcid.org/0000-0001-6122-1024)
- Bart Everts (ORCID: https://orcid.org/0000-0002-3764-5131)
Institutions
- Cairo University (EG)
- Leiden University Medical Center (NL)
- Netherlands Metabolomics Centre (NL)
- Delft University of Technology (NL)
Publication Details
- Journal
- Nature Methods
- Published
- 2024-08-29
- DOI
- https://doi.org/10.1038/s41592-024-02392-6
- Citations
- 47
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 10.99
Funders
- European Commission
- Universiteit Leiden
- ZonMw
- Nierstichting
- Novo Nordisk
- Leids Universitair Medisch Centrum
- Novo Nordisk Fonden