PHYTOCHEMICAL EVALUATION OF THE ANTIOXIDANT POTENTIAL OF OLEA FERRUGINEA ETHYL ACETATE FRACTION IN OXIDATIVE STRESS-INDUCED CARDIAC HYPERTROPHY RAT MODEL

Reactive oxygen species (ROS) are a common contributor to many diseases, particularly heart failure / cardiac hypertrophy. Phytochemicals are considered key components to counteract ROS and can be utilized in treatment without side effects. The study aimed at phytochemical screening of O. ferruginea ethyl acetate fraction (EAF) and evaluation of its antioxidant capacity using 2, 2-Diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) antioxidant assay, and in vivo oxidative stress-induced Cardiac Hypertrophy (CH) rat model. Results of this study showed strong potential for scavenging ROS with IC50 value 43.89±1.23 µg/ml) with DPPH and H2O2 along with the presence of higher Total Phenolic Content (TPC) (560 ±8.3), and Total Flavonoid Content (TFC) (430±4.7) contents. Gas Chromatography-Mass Spectrometry (GC-MS) analysis translated most of the compounds. Further in vitro results were validated by in vivo, an antiradical scavenging power estimation study on the oxidative stress-induced CH rat model with a significant reduction in H2O2 levels (3.5 fold) versus the diseased control group (7 fold) (p < 0.05).  This study concludes that O. ferruginea may serve as an anti-oxidant pool of natural active compou nds that can be utilized for further biochemical and molecular analysis to control Cardiac hypertrophy