Integration and validation of host transcript signatures, including a novel 3-transcript tuberculosis signature, to enable one-step multiclass diagnosis of childhood febrile disease
Our study demonstrates the feasibility of NanoString technology for cross-platform validation of multiple transcriptomic signatures in parallel. This external cohort validated performance of all five signatures, including a novel sparse TB signature. Two exploratory multi-class models showed high potential accuracy across four distinct diagnostic groups.
Authors
- Brian Eley (ORCID: https://orcid.org/0000-0003-0811-0098)
- Suzanne T. Anderson (ORCID: https://orcid.org/0000-0003-1213-7787)
- Dominic Habgood-Coote (ORCID: https://orcid.org/0000-0001-5501-0335)
- Andrew Brent (ORCID: https://orcid.org/0000-0002-5349-0723)
- Myrsini Kaforou (ORCID: https://orcid.org/0000-0001-9878-4007)
- Robert S. Heyderman (ORCID: https://orcid.org/0000-0003-4573-449X)
- Ortensia Vito (ORCID: https://orcid.org/0000-0001-5509-6351)
- Victoria Wright (ORCID: https://orcid.org/0000-0001-7826-1516)
- Samuel Channon‐Wells (ORCID: https://orcid.org/0000-0002-8166-8680)
- Federico Martinón‐Torres (ORCID: https://orcid.org/0000-0002-9023-581X)
- Jethro Herberg (ORCID: https://orcid.org/0000-0001-6941-6491)
- Rachel Galassini (ORCID: https://orcid.org/0000-0002-1025-0367)
- Michael Levin (ORCID: https://orcid.org/0000-0003-2767-6919)
Institutions
- University of Cape Town (ZA)
- Universidade de Santiago de Compostela (ES)
- Instituto de Salud Carlos III (ES)
- University of Oxford (GB)
- MRC Clinical Trials Unit at UCL (GB)
- Complejo Hospitalario Universitario de Santiago (ES)
- Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (ES)
- Oxford University Hospitals NHS Trust (GB)
- University College London (GB)
- Imperial College London (GB)
- Royal College of Paediatrics and Child Health (GB)
Publication Details
- Journal
- Journal of Translational Medicine
- Published
- 2024-08-29
- DOI
- https://doi.org/10.1186/s12967-024-05241-4
- Citations
- 5
- Primary Topic
- Tuberculosis Research and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.95
Funders
- Queen Mary University of London
- Wellcome Trust
- National Institute for Health and Care Research
- HORIZON EUROPE Framework Programme
- NIHR Imperial Biomedical Research Centre