Structural Analysis and Antioxidant Activity of Alkaline-Extracted Glucans from Hericium erinaceus

An alkali-soluble β-glucan (AHEP-A-b, 20 kDa) purified from <i>Hericium erinaceus</i> fruiting bodies, was structurally characterized and examined for antioxidant activity. Methylation analysis and NMR spectroscopy show that the backbone of AHEP-A-b is composed of (1→6)-linked-D-β-glucopyran residues, branched at O-3 of glucopyranose (Glc<i>p</i>) residues with [→3)-β-D-Glc<i>p</i>-(1→] oligosaccharides or single unit of β-Glc<i>p</i>. Periodate oxidation analysis and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) indicate that the degree of polymerization (DP) of [→3)-β-D-Glc<i>p</i>-(1→] side chains is 2 to 8. Functionally, AHEP-A-b is a relatively strong antioxidant as demonstrated by using 2, 2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical (ABTS^·+), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and hydroxyl radicals scavenging assays. The present study lays the foundation for further studies into structure-activity relationships of polysaccharides from <i>H. erinaceus</i>.