Mutant NPM1 marginally impacts ribosome footprint in acute myeloid leukemia cells
Incubation of degron cells with the small compound dTAG-13 enables highly specific degradation of NPM1c within 4 hours. As expected, RNA-sequencing data showed early loss of homeobox gene expression following NPM1c degradation, confirming the reliability of our model. In contrast, Ribo-seq data showed negligible changes in the ribosome footprint in both cell lines, implying that the presence of NPM1c does not influence ribosome abundance and positioning on mRNA. While it is predictable that NPM1c exerts its leukemogenic activity at multiple levels, ribosome footprint does not seem influenced by the presence of mutant NPM1.
Authors
- Brunangelo Falini (ORCID: https://orcid.org/0000-0002-7198-5965)
- Giulia Pianigiani (ORCID: https://orcid.org/0000-0003-0851-1473)
- Michael C. Gundry (ORCID: https://orcid.org/0000-0003-3654-1741)
- Margaret A. Goodell (ORCID: https://orcid.org/0000-0003-1111-2932)
- Lorenzo Brunetti (ORCID: https://orcid.org/0000-0003-2624-8576)
Institutions
- Marche Polytechnic University (IT)
- Baylor College of Medicine (US)
- University of Perugia (IT)
- IRCCS Materno Infantile Burlo Garofolo (IT)
- Regenerative Medicine Institute (MX)
Publication Details
- Journal
- eJHaem
- Published
- 2024-08-20
- DOI
- https://doi.org/10.1002/jha2.996
- Citations
- 2
- Primary Topic
- Acute Myeloid Leukemia Research
- Type
- article
- Field-Weighted Citation Impact
- 0.91
Funders
- Associazione Italiana per la Ricerca sul Cancro
- National Institutes of Health
- European Research Council