Effects of Gamma Irradiation on Changes in Chemical Composition and Antioxidant Activity of Euphorbia maculata Callus
In this study, we investigated the effects of gamma irradiation on the antioxidant activity and metabolite profiles of <i>Euphorbia maculata</i> calli (PC3012). Gamma irradiation at various doses (0, 0.05, 0.5, and 10 kGy) significantly enhanced the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-<i>bis</i>(3-ethylbenzothiazoline-6-sulphonic acid (ABTS<sup>+</sup>) radical scavenging activities of the callus extracts of PC3012 in a dose-dependent manner. High-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) analyses revealed that irradiation increased the lysophospholipid content, although no new antioxidant compounds were formed. Furthermore, a PLS-DA analysis revealed evident metabolic differences between non-irradiated and irradiated samples, which were further verified by statistical validation. These findings suggest that gamma irradiation induces specific biochemical modifications that enhance the bioactive properties of PC3012 calli. This technology exhibits potential for utilization in the natural product and food sectors, particularly in the development of functional foods and nutraceuticals with improved health benefits.
Authors
- Youngchul Yoo
- Sungbeom Lee (ORCID: https://orcid.org/0000-0003-3795-7597)
- Young Bae Ryu (ORCID: https://orcid.org/0000-0002-2683-7066)
- Seo Jun Lee
- Moon‐Soo Chung (ORCID: https://orcid.org/0000-0001-5414-5078)
- Hyoung‐Woo Bai (ORCID: https://orcid.org/0000-0002-1122-171X)
- Seung Sik Lee (ORCID: https://orcid.org/0000-0003-3900-9312)
- Gyeong Han Jeong (ORCID: https://orcid.org/0000-0001-7115-4945)
- Tae Hoon Kim (ORCID: https://orcid.org/0000-0003-0428-2829)
- Shubhpreet Kaur (ORCID: https://orcid.org/0000-0002-6297-472X)
- Jin‐Hong Kim (ORCID: https://orcid.org/0000-0003-2858-7512)
- Kwang‐Woo Jung (ORCID: https://orcid.org/0000-0003-0021-5100)
- Byung Yeoup Chung (ORCID: https://orcid.org/0000-0002-9777-3661)
Institutions
- Korea Atomic Energy Research Institute (KR)
- Korea Research Institute of Bioscience and Biotechnology (KR)
- Korea University of Science and Technology (KR)
- Daegu University (KR)
Publication Details
- Journal
- Plants
- Published
- 2024-08-19
- DOI
- https://doi.org/10.3390/plants13162306
- Citations
- 3
- Primary Topic
- Plant Genetic and Mutation Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.80
Funders
- Korea Atomic Energy Research Institute
- Ministry of Science and ICT, South Korea