Correlation between Klebsiella Species’ Biofilm Formation and Antibiotic Resistance from Erbil Hospital Patients

The primary cause of nosocomial infections is Klebsiella pneumonia, an environmental gram-negative, encapsulated, non-motile bacterium. Antibiotic resistance and biofilm formation are two crucial characteristics that contribute to the pathogenicity of this bacterium. This research aimed to examine the relationship between antibiotic resistance and the capability of Klebsiella species isolated from Erbil hospital patients to produce biofilms. Therefore, in this study, 38 samples were collected from different laboratories and hospitals. At first, the samples were rejuvenated in Tryptic soy broth and then cultured on MacConkey agar. Gram staining and urease test were performed to identify the bacterial isolates while VITEK 2 system was performed to determine both the bacterial isolates and the antibiotic susceptibility. At last, Biofilm detection was assessed by the microliter plate technique as well as the Congo red agar method. The results revealed positive Klebsiella samples that were both resistant to some antibiotics and biofilm producers.