Development and validation of a pentaplex PCR assay for rapid detection of blaCTX-M, blaOXA–1, blaCMY, blaNDM and the PBP3 insert in Enterobacterales
The pentaplex PCR was effective in detecting four beta-lactamases (bla<sub>CTX-M</sub>, bla<sub>OXA-1</sub>, bla<sub>CMY</sub>, bla<sub>NDM</sub>) as well as PBP3 inserts. It is expected that using pentaplex PCR as a diagnostic test for resistance detection in clinical practice will improve patient outcomes by providing prompt and targeted treatment.
Authors
- Subha Vajjiravelu Jaganathan
- Yamuna Devi Bakthavatchalam (ORCID: https://orcid.org/0000-0002-8840-3255)
- Binesh Lal Yesudason
- Kamini Walia (ORCID: https://orcid.org/0000-0002-1494-6919)
- Balaji Veeraraghavan (ORCID: https://orcid.org/0000-0002-8662-4257)
- Rema Devi
- Nivedhana Subburaju (ORCID: https://orcid.org/0009-0008-6461-3814)
- Ayyanraj Neeravi (ORCID: https://orcid.org/0009-0007-5891-8542)
- Sangeetha Nithiyanandam
- Poojah Shah
- Gita Nataraj
- Devishree Srinivasan
- Fizaa Abdullah
Institutions
- Christian Medical College, Vellore (IN)
- King Edward Memorial Hospital and Seth G.S. Medical College (IN)
- Dr. Somervell Memorial CSI Medical College and Hospital (IN)
- Indian Council of Medical Research (IN)
- Christian Medical College (IN)
- Meenakshi Medical College Hospital and Research Institute (IN)
- Rainbow Children's Hospital (IN)
Publication Details
- Journal
- Indian Journal of Medical Microbiology
- Published
- 2024-08-30
- DOI
- https://doi.org/10.1016/j.ijmmb.2024.100710
- Citations
- 1
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.17