Experimental visualization of optical spatial sensitivity through combination of diffuse correlation spectroscopy and acoustic radiation force

In field of diffuse optics for biomedical applications, the spatial sensitivity (SS) is a key parameter to evaluate or optimize the adopted modalities, such as penetration depth, signal-to-noise ratio as well as sensor distribution. Nevertheless, SS is usually estimated via computer simulations (e.g., photon Monte Carlo simulation), rather than being quantified experimentally, due to the technical difficulty. In this study, we report the experimental measurement and visualization of optical SS through combination of acoustic radiation force (ARF) and the scanning diffuse correlation spectroscopy (DCS). By spatially varying the location of ARF focal spot within liquid phantom, the enhanced particle flow, which represents the most spatial sensitive location, was identified by DCS. The experimental outcomes were cross-validated with the photon Monte Carlo simulation, thus demonstrating its accuracy, feasibility, and potential for guiding clinical usage.