Comparison of different parts of tangerine (Citrus tangerina) for DNA extraction

The tangerine, a citrus fruit, is grown commercially worldwide in tropical, semitropical, and warm temperate countries, including the Mediterranean region. Tangerine cultivation and production are crucial for the economy of many countries, where genomic DNA plays a critical role in genetic diversity and molecular analysis of this tree. Therefore, this study was conducted in 2022 at Limpopo University to compare DNA extraction from three different parts of the tangerine tree: fresh leaves, stems, and fruit. The Chelex method was used to extract DNA from three samples of C. tangerine from South Africa. The quantity and purity of DNA were measured using a spectrophotometer. PCR amplification with 28S primers was used to confirm and evaluate the extracted DNA. The results from the spectrophotometer showed that the highest quantity of nucleic acid was extracted from the stem (98.18± 12 mg/mL), with a 260/280 ratio for DNA quality (1.85 ± 0.01). The fruit showed a high level of protein (5.8 ± 1.8 mg/mL), but the DNA concentration was lower. In conclusion the amplification of the samples indicated that the stem had high purity and concentration of DNA, which can be suitable for PCR amplification.