Fraggler: A Python Package and CLI Tool for AutomatedFragment Analysis

Fragment Analysis (FA) is a PCR based technique which separates DNA fragments according to their sizes using a capillary electrophoresis instrument.PCR products are marked by fluorescent dyes and the intensities and migration time of the emitted signal are measured (Covarrubias-Pazaran et al., 2016;van Steenderen, 2022).This enables a multitude of applications including Sanger sequencing, microsatellite marker analysis, multiplex ligation-dependent probe amplification assays, and more.