Spatial light modulation for interferometric scattering microscopy
Interferometric scattering (iSCAT) microscopy enables high-speed and label-free detection of individual molecules and small nanoparticles. Here we apply point spread function engineering to provide adaptive control of iSCAT images using spatial light modulation. With this approach, we demonstrate improved dynamic spatial filtering, real-time background subtraction, focus control, and signal modulation based on sample orientation.
Authors
- Christopher Parperis
- Mark I. Wallace (ORCID: https://orcid.org/0000-0002-5692-8313)
- Vivien Walter (ORCID: https://orcid.org/0000-0003-3203-9543)
- Yujie Guo (ORCID: https://orcid.org/0009-0003-8411-8400)
Institutions
- King's College London (GB)
Publication Details
- Journal
- Journal of Microscopy
- Published
- 2024-08-26
- DOI
- https://doi.org/10.1111/jmi.13347
- Citations
- 2
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.32
Funders
- Wellcome Trust
- Oxford Nanopore Technologies
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council