Visualization of Brain Tumors with Infrared-Labeled Aptamers for Fluorescence-Guided Surgery
Gliomas remain challenging brain tumors to treat due to their infiltrative nature. Accurately identifying tumor boundaries during surgery is crucial for successful resection. This study introduces an innovative intraoperative visualization method utilizing surgical fluorescence microscopy to precisely locate tumor cell dissemination. Here, the focus is on the development of a novel contrasting agent (IR-Glint) for intraoperative visualization of human glial tumors comprising infrared-labeled Glint aptamers. The specificity of IR-Glint is assessed using flow cytometry and microscopy on primary cell cultures. In vivo effectiveness is studied on mouse and rabbit models, employing orthotopic xenotransplantation of human brain gliomas with various imaging techniques, including PET/CT, in vivo fluorescence visualization, confocal laser scanning, and surgical microscopy. The experiments validate the potential of IR-Glint for the intraoperative visualization of gliomas using infrared imaging. IR-Glint penetrates the blood-brain barrier and can be used for both intravenous and surface applications, allowing clear visualization of the tumor. The surface application directly to the brain reduces the dosage required and mitigates potential toxic effects on the patient. The research shows the potential of infrared dye-labeled aptamers for accurately visualizing glial tumors during brain surgery. This novel aptamer-assisted fluorescence-guided surgery (AptaFGS) may pave the way for future advancements in the field of neurosurgery.
Authors
- D.S. Grek
- P. G. Shnyakin (ORCID: https://orcid.org/0000-0001-6321-4557)
- Galina S. Zamay (ORCID: https://orcid.org/0000-0002-2567-6918)
- Кirill V. Belugin (ORCID: https://orcid.org/0000-0003-3465-104X)
- Maxim V. Berezovski (ORCID: https://orcid.org/0000-0003-0514-599X)
- Natalia A. Luzan (ORCID: https://orcid.org/0009-0001-8983-5017)
- Olga S. Kolovskaya (ORCID: https://orcid.org/0000-0002-2494-2313)
- Vadim Kumeiko (ORCID: https://orcid.org/0000-0002-1639-664X)
- Olga Kuziakova (ORCID: https://orcid.org/0000-0002-3799-1691)
- Anastasia A. Koshmanova (ORCID: https://orcid.org/0000-0001-7339-8660)
- Elena D. Nikolaeva (ORCID: https://orcid.org/0000-0002-5298-828X)
- Е. В. Семичев (ORCID: https://orcid.org/0000-0003-2386-5798)
- Irina A. Shchugoreva (ORCID: https://orcid.org/0000-0003-4207-1627)
- Victoriya D. Fedotovskaya (ORCID: https://orcid.org/0000-0002-6472-0782)
- Kirill A. Lukyanenko (ORCID: https://orcid.org/0000-0002-1115-6735)
- Daria A. Kirichenko (ORCID: https://orcid.org/0000-0003-4087-731X)
- Polina V. Artyushenko (ORCID: https://orcid.org/0000-0002-3176-8202)
- А. К. Кириченко (ORCID: https://orcid.org/0000-0001-5416-7904)
- В. И. Бахтина (ORCID: https://orcid.org/0000-0002-6465-9942)
- Nikolay А. Tokarev (ORCID: https://orcid.org/0000-0002-3335-0547)
- А. А. Народов (ORCID: https://orcid.org/0000-0001-7384-0682)
- A. R. Gorbushin (ORCID: https://orcid.org/0000-0003-4186-588X)
- В. А. Светличный (ORCID: https://orcid.org/0000-0002-3935-0871)
- И. Н. Лапин (ORCID: https://orcid.org/0000-0001-5736-3791)
- Yury E. Glazyrin (ORCID: https://orcid.org/0000-0002-2826-5751)
- P. А. Shesternya (ORCID: https://orcid.org/0000-0001-8652-1410)
- Dmitry V. Veprintsev
- Vasily S. Mezko
- Р. А. Зуков (ORCID: https://orcid.org/0000-0002-7210-3020)
- N. G. Chanchikova (ORCID: https://orcid.org/0000-0002-7079-4067)
- Vladimir А. Khorzhevskii (ORCID: https://orcid.org/0000-0002-9196-7246)
- Felix N. Tomilin (ORCID: https://orcid.org/0000-0002-3578-6141)
- Alexey A. Mohov
- Tatiana N. Zamay
- Aleksandra Kosinova
- Anna Kichkailo
- Alexey V. Krat
- Ivan I. Voronkovskii
Institutions
- University of Ottawa (CA)
- National Research Tomsk State University (RU)
- Far Eastern Federal University (RU)
- Krasnoyarsk Scientific Center (RU)
- Institute of Chemistry and Chemical Technology (RU)
- Krasnoyarsk State Medical University (RU)
- Advanced Materials Technology (United States) (US)
- Institute of Physics (RU)
- Krasnoyarsk Regional Clinical Hospital (RU)
- TechLab (United States) (US)
- A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch of the Russian Academy of Sciences (RU)
- Far Eastern Branch of the Russian Academy of Sciences (RU)
- Institute of Physics of the Slovak Academy of Sciences (SK)
- Federal Medical-Biological Agency (RU)
Publication Details
- Journal
- Journal of the American Chemical Society
- Published
- 2024-08-26
- DOI
- https://doi.org/10.1021/jacs.4c06716
- Citations
- 11
- Primary Topic
- Nanoplatforms for cancer theranostics
- Type
- article
- Field-Weighted Citation Impact
- 2.03
Funders
- University of Ottawa
- Siberian Branch, Russian Academy of Sciences
- Ministry of Science and Higher Education of the Russian Federation
- Ministry of Health of the Russian Federation
- Natural Sciences and Engineering Research Council of Canada
- Russian Academy of Sciences