Comparative Analysis of Digestion Methods for Bile Proteomics: The Key to Unlocking Biliary Biomarker Potential
Variability between methods emphasizes the importance of cross-validation across multiple analytical approaches to ensure robust analysis. We recommend the in-gel crude method for its simplicity and efficiency, avoiding additional precipitation steps. Sample processing speed, cost, cleanliness, and reproducibility should be considered when a digestion method is selected for bile proteomics.
Authors
- Folkert Kuipers (ORCID: https://orcid.org/0000-0003-2518-737X)
- Vincent Erwin de Meijer (ORCID: https://orcid.org/0000-0002-7900-5917)
- Robert Jack Porte (ORCID: https://orcid.org/0000-0003-0538-734X)
- Adam M. Thorne (ORCID: https://orcid.org/0000-0001-5550-2364)
- Justina C. Wolters (ORCID: https://orcid.org/0000-0003-0066-3720)
- Martijn Hoekzema
Institutions
- University Medical Center Groningen (NL)
- University of Groningen (NL)
- Hanze University of Applied Sciences (NL)
- Erasmus MC (NL)
- Dialyse Centrum Groningen (NL)
- Erasmus MC Transplant Institute
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2024-08-26
- DOI
- https://doi.org/10.1021/acs.analchem.4c01766
- Primary Topic
- Organ Transplantation Techniques and Outcomes
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Nederlandse Vereniging voor Gastroenterologie
- Noaber Foundation
- Ministerie van Economische Zaken
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek