Latency period and ovulation rate of Pangasius hypophthalmus after the induction of different doses of ovaprim breeding hormone

Pangasius hypophthalmus (Sauvage, 1878), native to the Mekong River, has the potential to fulfill the protein requirements of the most populous and developing country, Pakistan. The objectives of this research were to determine the optimal dosage of ovaprim for increasing the ovulation rate in female P. hypophthalmus and to investigate its impact on the latency period. There were two pairs of male–female fish in each of the three groups, and each group of fish was placed in separate cement tanks. Our study included administering three distinct dosages of ovaprim to three separate groups of male and female P. hypophthalmus. We then measured their body weights and ovulation cycles. In Group 1, females were administered a dosage of 0.5 ml/kg on the basis of their body weight, and their ovulation phase lasted for 13–14 hours. In Group 2, a dose of 0.6 ml/kg was administered to females and their ovulation duration resided for 13–15 hours. Group 3, on the other hand, was administered a dosage of 0.7 ml/kg to females, and the ovulation period for this group lasted 12–14 hours. In contrast, males in all three groups were given an OVP injection of 0.2 ml/kg b.w. The latency period, ovulation duration, and fertilization rate of all female P. hypophthalmus samples were approximately 16–18 h, 12–15 h, and 42–65%, respectively. Our findings revealed that a single dose of OVP (0.7 ml/kg b.w.) was effective. It was concluded that OVP may be used by hatchery operators to artificially promote maximum spawning of P. hypophthalmus.