Aspects of protease from isolated bacteria from bovine milk

Ninety-five isolates were screened for produce of protease enzymes. The most potent isolate was identified as Bacillus sp. The aim of study is production and characterization of protease from selected isolate. Protease production was increased by optimizing the culture conditions, these included physical parameters like incubation time, pH, and inoculum size. Lactose and yeast extract were the best carbon and nitrogen sources. The purified enzyme was found to be stable at pH 5–9. The activity of protease increased under an alkaline condition with the optimum pH estimated at pH 9 and thermostability at 50°C. Partially purified protease enzymes were inhibited with EDTA and SDS. EDTA concentrations show a strong inhibitory effect on protease activity. The effect of various metal ions at concentrations of 1, 5, 10, and 15 mM on crude protease was investigated Protease enzyme activated with Ca, Ni, Co, and Mn. Result shows a strong positive correlation between Ni concentration and protease activity.