New Iflavirus Species Characterized from Mosquitoes Captured in the Sao Paulo Zoological Facilities
Metagenomic studies of mosquito viromes demonstrated a more diverse composition than just an exclusive composition of pathogenic arboviruses transmitted to humans. In our study, the virome of 866 female mosquitoes collected throughout 2020 at the São Paulo Zoo, located in the city of São Paulo/SP-Brazil, was obtained. Specifically, in this paper, we describe a new virus found by viral RNA extraction and next-generation MiSeq sequencing of a group of 23 specimens of <i>Anopheles</i> (<i>Nys</i>.) <i>strodei</i>. The complete genome with a length of 9709 nucleotides was characterized by a positive orientation and a single strand, with a single large ORF, which encodes a polyprotein of 2987 amino acids. The phylogenetic analysis showed an association with the viral family <i>Iflaviridae</i> and the <i>Riboviria</i> realm. We carried out comparisons with translated sequences of the capsid regions of other iflavirus, and the identities in relation to our sequence were below the minimum limit of 90%, indicating that possibly it is a new species of iflavirus. Our findings contribute to expanding knowledge of virome composition among mosquito species in Brazil and globally. Moreover, we provide a viral genome reference specific to this geographic region and <i>Culicidae</i> family of mosquitoes. This resource facilitates future in silico recognition and assembly of viral genomes within metagenomic datasets.
Authors
- Élcio Leal (ORCID: https://orcid.org/0000-0003-3463-0492)
- Lilian de Oliveira Guimarães (ORCID: https://orcid.org/0000-0001-6535-2988)
- Santana Lobato Bahia
- Vera Lucia Fonseca de Camargo-Neves (ORCID: https://orcid.org/0000-0001-8287-7727)
- Ramendra Pati Pandey (ORCID: https://orcid.org/0000-0002-5196-6468)
- Juliana Telles-de-Deus
- Endrya do Socorro Fôro Ramos (ORCID: https://orcid.org/0000-0001-5229-896X)
- Vanessa dos Santos Morais (ORCID: https://orcid.org/0000-0002-3813-3154)
- Vanessa Christe Helfstein (ORCID: https://orcid.org/0009-0008-4621-385X)
- Geovani de Oliveira Ribeiro (ORCID: https://orcid.org/0000-0002-8832-7803)
- Eric Delwart (ORCID: https://orcid.org/0000-0002-6296-4484)
- Fabiola Villanova (ORCID: https://orcid.org/0000-0003-4442-5855)
- Karin Kirchgatter (ORCID: https://orcid.org/0000-0002-2449-2316)
- Antônio Charlys da Costa (ORCID: https://orcid.org/0000-0001-5516-5177)
- Xutao Deng
- Jesus Maia dos Santos
Institutions
- Universidade de Brasília (BR)
- Universidade de São Paulo (BR)
- University of California, San Francisco (US)
- Pacific Research Institute (US)
- Ministério da Saúde (BR)
- Vitalant (US)
- Instituto Pasteur (BR)
- University of Petroleum and Energy Studies (IN)
- Universidade Federal do Pará (BR)
Publication Details
- Journal
- Microorganisms
- Published
- 2024-08-23
- DOI
- https://doi.org/10.3390/microorganisms12091749
- Citations
- 6
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 3.26
Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Ministry of Environment
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Universidade de São Paulo