Antitumor activity of protein fraction of Jatropha curcas In DMBA and UV B induced skin tumors in mice: Expression of Bcl-2

UV radiation and 7,12-dimethylbenz[α]anthracene (DMBA) induced skin tumors can be seen from the decreased Bcl-2 as a suppressor gene regulating programmed cell death and apoptosis. This study aims to determine the antitumor activity of protein fractions from Jatropha curcas leaves against skin tumor cells through the expression of Bcl-2 protein in mice-induced DMBA and UVB. This study used 40 mice divided into 4 groups. Group 1 was the control group without treatment. Group 2 was induced 0.2% DMBA (200μl/mice) and UVB (5x/week) for 8 weeks. Groups 3 and 4 were induced 0.2% DMBA (200μl/mice) and UVB (5x/week) for 8 weeks of continued treatment with DMSO, and the protein fraction dose of 2 mg/0,1 ml for 4 weeks, respectively. Immunohistochemistry examination from the tissue skin of mice was performed by using Bcl-2 monoclonal antibody on week 8 (after induced UVB) and on week 12 (after treatment). The results showed that Bcl-2 expression was significantly different in group 2 (induced DMBA and UVB) and group 4 (protein fraction) (p<0,05). Expression of Bcl-2 was found to be lowest in the group with treatment by protein fraction dose of 2 mg/0,1 ml. The highest expression was found in the group with induced DMBA and UVB. Decreased expression of Bcl-2 is a sign of apoptosis, so the protein Jatropha curcas has antitumor through induction of apoptosis.