Cross comparison of alternative diagnostic protocols including substitution to the clinical sample, RNA extraction method and nucleic acid amplification technology for COVID-19 diagnosis
our comparison shows that RT-LAMP technology has diagnostic performance on par with RT-qPCR; likewise, saliva offers the same diagnostic functionality as NPS when subjected to a TRE method. Nonetheless, use of direct saliva after a HIRR and detected with RT-LAMP does not produce an acceptable diagnostic performance.
Authors
- Jordi Querol‐Audí (ORCID: https://orcid.org/0000-0003-2892-7599)
- Ismael Segura-Ulate (ORCID: https://orcid.org/0000-0002-6069-6252)
- German Madrigal Redondo (ORCID: https://orcid.org/0000-0002-9856-4044)
- Navilla Apú (ORCID: https://orcid.org/0000-0002-6848-7252)
- Andrés Gatica-Arias (ORCID: https://orcid.org/0000-0002-3841-0238)
- Fernando Flores-Mora
- Bernal Cortés
- C Ortega (ORCID: https://orcid.org/0009-0006-4031-5922)
- Yamitzel Zaldívar
Institutions
- Universidad de Panamá (PA)
- Instituto Conmemorativo Gorgas de Estudios de la Salud (PA)
- Universidad de Costa Rica (CR)
- Instituto Costarricense de Investigación y Enseñanza en Nutrición y Salud (CR)
- Secretaría Nacional de Ciencia, Tecnología e Innovación (PA)
- University of El Salvador (SV)
Publication Details
- Journal
- Frontiers in Molecular Biosciences
- Published
- 2024-08-23
- DOI
- https://doi.org/10.3389/fmolb.2024.1445142
- Primary Topic
- Biosensors and Analytical Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Consejo Superior Universitario Centroamericano
- Universidad de Costa Rica
- Austrian Centre of Industrial Biotechnology