Protocol for quantifying drug sensitivity in 3D patient-derived ovarian cancer models
Three-dimensional (3D) ex vivo cultures allow the study of cancer progression and drug resistance mechanisms. Here, we present a protocol for measuring on-target drug sensitivity in a scaffold-free 3D culture system through quantification of apoptotic tumor cells. We provide detailed steps for sample processing, immunofluorescence staining, semi-high-throughput confocal imaging, and imaged-based quantification of 3D cultures. This protocol is versatile and can be applied in principle to any patient-derived material.
Authors
- André Fedier (ORCID: https://orcid.org/0000-0002-5613-8629)
- Ricardo Coelho (ORCID: https://orcid.org/0000-0002-9340-3612)
- Flavio C. Lombardo (ORCID: https://orcid.org/0000-0002-4853-6838)
- Francis Jacob (ORCID: https://orcid.org/0000-0002-0446-1942)
- Mónica Núñez López
- Viola A Heinzelmann-Schwarz (ORCID: https://orcid.org/0000-0002-5613-8629)
- Natalie Rimmer
- Kathrin B. Labrosse (ORCID: https://orcid.org/0000-0002-7195-5360)
Institutions
- University of Basel (CH)
- University Hospital of Basel (CH)
- Department of Biomedicine Basel (CH)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-08-23
- DOI
- https://doi.org/10.1016/j.xpro.2024.103274
- Citations
- 3
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.74
Funders
- National Science Foundation
- Universität Basel
- Wilhelm Sander-Stiftung
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Krebsliga Schweiz
- Swiss Cancer Research Foundation