The dual role of casein kinase 1, DTG1, in regulating tillering and grain size in rice
Tiller number and grain size are important agronomic traits that determine grain yield in rice. Here, we demonstrate that DEFECTIVE TILLER GROWTH 1 (DTG1), a member of the casein kinase 1 protein family, exerts a co-regulatory effect on tiller number and grain size. We identified a single amino acid substitution in DTG1 (I357K) that caused a decrease in tiller number and an increase in grain size in NIL- dtg1 . Genetic analyses revealed that DTG1 plays a pivotal role in regulation of tillering and grain size. The DTG1 I357K allelic variant exhibited robust functionality in suppressing tillering. We show that DTG1 is preferentially expressed in tiller buds and young panicles, and negatively regulates grain size by restricting cell proliferation in spikelet hulls. We further confirm that DTG1 functioned in grain size regulation by directly interacting with Grain Width 2 (GW2), a critical grain size regulator in rice. The CRISPR/Cas9-mediated elimination of DTG1 significantly enhanced tiller number and grain size, thereby increasing rice grain yield under field conditions, thus highlighting potential value of DTG1 in rice breeding.
Authors
- Genxu Wang (ORCID: https://orcid.org/0000-0002-3403-0983)
- Jinghua Jin
- Ting Zou (ORCID: https://orcid.org/0000-0002-6442-4895)
- Dan Zhou (ORCID: https://orcid.org/0000-0003-0426-0339)
- Deke Li (ORCID: https://orcid.org/0000-0001-7171-6389)
- Yunqing Tong
- Yueyang Liang (ORCID: https://orcid.org/0000-0002-7427-1028)
- Jijin Li
- Chenglong Jiang
- Keke Liu (ORCID: https://orcid.org/0009-0000-4572-7690)
- Shuangcheng Li (ORCID: https://orcid.org/0000-0001-5684-0533)
- Haipeng Wang (ORCID: https://orcid.org/0000-0003-1912-7143)
- Ping Li (ORCID: https://orcid.org/0000-0002-1558-9888)
- Kang Chen (ORCID: https://orcid.org/0009-0001-9101-8964)
- Lingfeng Li (ORCID: https://orcid.org/0000-0001-9141-8397)
- Qiming Deng (ORCID: https://orcid.org/0000-0001-9045-277X)
- Jun Zhu (ORCID: https://orcid.org/0000-0002-9427-4517)
- Rui Qin (ORCID: https://orcid.org/0000-0003-4093-7044)
- Binjiu Luo
- Shiquan Wang (ORCID: https://orcid.org/0000-0003-3163-2476)
- Jiangkun Ye
- Chengqin Gong
Institutions
- North Sichuan Medical University (CN)
- Sichuan Agricultural University (CN)
- Rice Research Institute (CN)
- Neijiang Normal University (CN)
- Nanchong Central Hospital (CN)
- Sichuan Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- The Crop Journal
- Published
- 2024-08-23
- DOI
- https://doi.org/10.1016/j.cj.2024.07.016
- Citations
- 1
- Primary Topic
- Genetic Mapping and Diversity in Plants and Animals
- Type
- article
- Field-Weighted Citation Impact
- 0.55
Funders
- National Natural Science Foundation of China
- Sichuan Province Science and Technology Support Program