COMPARASION OF HUMAN MEMBRANE AMNION USING SODIUM DODECYL SULFATE (SDS) DECELLULARIZATION OUTCOME AND COMBINATION OF SDS WITH DNase ENZYME

Introduction.Decellularized human amnion membrane (dHAM) has a lower risk of immunogenic inflammatory response than human amnion membrane (HAM) without decellularization.Decellularized human amnion membrane can accelerates the wound healing process by preserving skin structure, making wound recovery using stem cells faster than without dHAM.This research aims to prove that dHAM can be made using 0.5% SDS alone or in combination with DNase while maintaining the collagen component of HAM.Methods.This research is an in vitro experimental study that uses HAM from the birthing process in a hospital.This study consisted of one control group of freeze-dried HAM which was not decellularized, as well as four treatment groups underwent the decellularization process using SDS 0.5% for 15 minutes (dHAM 1) and SDS 0.5% for 30 minutes (dHAM 2), SDS 0.5 % for 15 minutes and DNase for 15 minutes (dHAM 3), SDS 0.5% for 30 minutes and DNase for 15 minutes (dHAM 4).The variables assessed were differences in cellular and collagen components, between the control and the treatment group, which were evaluated using an optical microscope.Results.Histological examination per field of view at 400x magnification of optical microscope showed that the decellularization process carried out was effective in reducing the number of cells in HAM (p < 0.0001; CI = 95%), the control group still appeared hypercellular (41.57 6.1 cells) , dHAM 1 appeared medium cellular (24.86 7.08 cells), dHAM 2 and dHAM 3 appeared hypocellular with almost the same effectiveness (dHAM 2 = 1 1 cells; dHAM 3 = 1.28 0.49 cells) , dHAM showed hypocellular results close to zero (0.28 0.49 cells).The collagen component showed almost the same results in the control group, dHAM 1, dHAM 2, dHAM 3, but the collagen component decreased in dHAM 4 (p < 0.0001; CI = 95%). Conclusion.Making dHAM with just 0.5% SDS takes a minimum of 30 minutes.The duration of SDS 0.5% can be reduced when combined with DNase.Increasing the duration of 0.5% SDS does not reduce the density of the HAM collagen component.However, increasing the duration of 0.5% SDS combined with DNase can reduce the density of HAM collagen.