Novel Self-Powered Biosensor Based on Au Nanoparticles @ Pd Nanorings and Catalytic Hairpin Assembly for Ultrasensitive miRNA-21 Assay
An ultrasensitive self-powered biosensor is constructed for miRNA-21 detection based on Au nanoparticles @ Pd nanorings (Au NPs@Pd NRs) and catalytic hairpin assembly (CHA). The Au NPs@Pd NRs possess excellent electrical conductivity to improve the electron transfer rate and show good elimination of byproduct H<sub>2</sub>O<sub>2</sub> to assist glucose oxidase (GOD) to catalyze glucose; CHA is used as an amplification strategy to effectively enhance the sensitivity of the biosensor. To further amplify the output signal, a capacitor is integrated into the self-powered biosensor. With multiple signal amplification strategies, the self-powered biosensor possesses a linear range of 0.1-10<sup>-4</sup> fM and a lower limit of detection (LOD) of 0.032 fM (<i>S</i>/<i>N</i> = 3). In addition, the as-prepared self-powered biosensor displays potential applicability in the assay toward miRNA-21 in human serum samples.
Authors
- Weihong Tan (ORCID: https://orcid.org/0000-0002-8066-1524)
- Futing Wang (ORCID: https://orcid.org/0000-0001-7306-5495)
- Jingxian Li (ORCID: https://orcid.org/0000-0003-1087-5428)
- Yujin Li (ORCID: https://orcid.org/0000-0003-1103-3273)
- Hongfen Yang (ORCID: https://orcid.org/0000-0001-8367-4104)
- Ren Cai (ORCID: https://orcid.org/0000-0003-2583-7133)
- Yan Shen (ORCID: https://orcid.org/0000-0002-9744-3224)
- Xiang Shi (ORCID: https://orcid.org/0000-0003-1963-3337)
- Yan Yang (ORCID: https://orcid.org/0009-0006-7177-1111)
Institutions
- Xinyang Normal University (CN)
- Hunan University (CN)
- Shanghai Jiao Tong University (CN)
- Renji Hospital (CN)
- Zhejiang Cancer Hospital (CN)
- Cancer Hospital of Chinese Academy of Medical Sciences (CN)
- University of Chinese Academy of Sciences (CN)
- University of South China (CN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2024-08-23
- DOI
- https://doi.org/10.1021/acs.analchem.4c02751
- Citations
- 18
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 4.21
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Hunan Province