Synthetic Nicotinamide Cofactors as Alternatives to NADPH in Imine Reductase-Catalyzed Reactions
This study demonstrates the effectiveness of synthetic nicotinamide cofactors as cost-effective alternatives to NADPH in imine reductase (IRED) catalysis. The synthetic cofactors maintained catalytic activity and stereoselectivity, achieving high conversion rates. Molecular docking studies revealed key structural interactions influencing performance. Combining a glucose dehydrogenase (GDH) recycling system further enhanced the stability and efficiency. These findings highlight the potential of synthetic cofactors to reduce costs and improve the feasibility of IRED-catalyzed processes for industrial applications.
Authors
- Yaqing Ma (ORCID: https://orcid.org/0009-0001-4766-2592)
- Shu‐Shan Gao (ORCID: https://orcid.org/0000-0001-6335-2052)
- Pingyuan Wang (ORCID: https://orcid.org/0009-0005-0368-5070)
- Chengsen Cui (ORCID: https://orcid.org/0000-0002-6867-1826)
- Chang‐Yun Wang (ORCID: https://orcid.org/0000-0002-0236-1606)
- Wei Kang (ORCID: https://orcid.org/0000-0002-7994-3524)
- Jinping Bao
- Lujia Yang (ORCID: https://orcid.org/0009-0005-7539-1936)
Institutions
- Chinese Academy of Sciences (CN)
- Qingdao National Laboratory for Marine Science and Technology (CN)
- Tianjin Institute of Industrial Biotechnology (CN)
- Ocean University of China (CN)
Publication Details
- Journal
- Organic Letters
- Published
- 2024-08-23
- DOI
- https://doi.org/10.1021/acs.orglett.4c02560
- Citations
- 4
- Primary Topic
- Enzyme Catalysis and Immobilization
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- National Key Research and Development Program of China