Nanoscale Surface Chemical Patterning of Soft Polyacrylamide with Elastic Modulus Similar to Soft Tissue

Nanometer-scale control over surface functionalization of soft gels is important for a variety of applications including controlling interactions with cells for <i>in vitro</i> cell culture and for regenerative medicine. Recently, we have shown that it is possible to transfer a nanometer-thick precision functional polymer layer to the surface of relatively stiff polyacrylamide gels. Here, we develop a fundamental understanding of the way in which the precision polymer backbone participates in the polyacrylamide radical polymerization and cross-linking process, which enables us to generate high-efficiency transfer to much softer hydrogels (down to 5 kPa) with stiffness similar to that of soft tissue. This approach creates hydrogel surfaces with exposed nanostructured functional arrays that open the door to controlled ligand presentation on soft hydrogel surfaces.