Atriplex hortensis var. 'rubra' extracts and purified amaranthin-type pigments reduce oxidative stress and inflammatory response in LPS-stimulated RAW264.7 cells
The use of direct injection ion mobility mass spectrometry (DI-IM-MS) to detect and identify betacyanin pigments in A. hortensis 'rubra' extracts was explored for the first time, with results compared to conventional LC-MS/MS analysis. The anti-inflammatory activities of leaf and seed extracts, alongside purified amaranthin and celosianin pigments, were investigated using a model of lipopolysaccharide (LPS)-activated murine macrophages. Extracts and purified pigments significantly inhibited the production of prostaglandin E<sub>2</sub> and NO by up to 90% and 70%, respectively, and reduced the expression of Il6, Il1b, Nos2, and Cox2. Leaf and seed extracts also decreased secretion of Il6 and Il1b cytokines and reduced protein levels of Nos2 and Cox2. Furthermore, extracts and purified pigments demonstrated potent dose-dependent radical scavenging activity in a cellular antioxidant activity assay (CAA) without any cytotoxic effects. Our research highlights the promising biological potential of edible, climate-resilient A. hortensis 'rubra' as a valuable source of bioactive compounds.
Authors
- Andrew C.W. Zannettino (ORCID: https://orcid.org/0000-0002-6646-6167)
- Tara Louise Pukala (ORCID: https://orcid.org/0000-0001-7391-1436)
- Sławomir Wybraniec (ORCID: https://orcid.org/0000-0002-1263-4188)
- Jvaughn Duggan
- River J. Pachulicz (ORCID: https://orcid.org/0000-0001-8730-7926)
- Agnieszka Kumorkiewicz‐Jamro (ORCID: https://orcid.org/0000-0002-3828-3128)
- Kate Vandyke (ORCID: https://orcid.org/0000-0002-1033-849X)
- Renata Górska (ORCID: https://orcid.org/0000-0002-6151-1811)
- Stephen Fitter (ORCID: https://orcid.org/0000-0003-1663-6807)
Institutions
- Cracow University of Technology (PL)
- South Australian Health and Medical Research Institute (AU)
- The University of Adelaide (AU)
Publication Details
- Journal
- Food Chemistry
- Published
- 2024-08-23
- DOI
- https://doi.org/10.1016/j.foodchem.2024.140920
- Citations
- 10
- Primary Topic
- Botanical Research and Applications
- Type
- article
- Field-Weighted Citation Impact
- 3.48
Funders
- Narodowe Centrum Nauki
- Narodowa Agencja Wymiany Akademickiej
- Department for Innovation and Skills, Government of South Australia