Genomic Functional Analysis of Novel Radiation-Resistant Species of Knollia sp. nov. S7-12T from the North Slope of Mount Everest

Radiation protection is an important field of study, as it relates to human health and environmental safety. Radiation-resistance mechanisms in extremophiles are a research hotspot, as this knowledge has great application value in bioremediation and development of anti-radiation drugs. Mount Everest, an extreme environment of high radiation exposure, harbors many bacterial strains resistant to radiation. However, owing to the difficulties in studying them because of the extreme terrain, many remain unexplored. In this study, a novel species (herein, S7-12^T) was isolated from the moraine of Mount Everest, and its morphology and functional and genomic characteristics were analyzed. The strain S7-12^T is white in color, smooth and rounded, non-spore-forming, and non-motile and can survive at a UV intensity of 1000 J/m², showing that it is twice as resistant to radiation as <i>Deinococcus radiodurans</i>. Radiation-resistance genes, including <i>IbpA</i> and those from the <i>rec</i> and <i>CspA</i> gene families, were identified. The polyphasic taxonomic approach revealed that the strain S7-12^T (=KCTC 59114T =GDMCC 1.3458T) is a new species of the genus <i>Knoellia</i> and is thus proposed to be named <i>glaciei</i>. The in-depth study of the genome of strain S7-12^T will enable us to gain further insights into its potential use in radiation resistance. Understanding how microorganisms resist radiation damage could reveal potential biomarkers and therapeutic targets, leading to the discovery of potent anti-radiation compounds, thereby improving human resistance to the threat of radiation.