Salmonella re-engineers the intestinal environment to break colonization resistance in the presence of a compositionally intact microbiota
The gut microbiota prevents harmful microbes from entering the body, a function known as colonization resistance. The enteric pathogen Salmonella enterica serovar (S.) Typhimurium uses its virulence factors to break colonization resistance through unknown mechanisms. Using metabolite profiling and genetic analysis, we show that the initial rise in luminal pathogen abundance was powered by a combination of aerobic respiration and mixed acid fermentation of simple sugars, such as glucose, which resulted in their depletion from the metabolome. The initial rise in the abundance of the pathogen in the feces coincided with a reduction in the cecal concentrations of acetate and butyrate and an increase in epithelial oxygenation. Notably, these changes in the host environment preceded changes in the microbiota composition. We conclude that changes in the host environment can weaken colonization resistance even in the absence of overt compositional changes in the gut microbiota.
Authors
- Derek J. Bays (ORCID: https://orcid.org/0000-0003-0264-5569)
- Michael L. Goodson (ORCID: https://orcid.org/0000-0002-3464-490X)
- Scott P. Mahan
- Thaynara Parente de Carvalho (ORCID: https://orcid.org/0000-0002-7158-8249)
- Jee‐Yon Lee (ORCID: https://orcid.org/0000-0002-6376-2681)
- Kohei Yamazaki (ORCID: https://orcid.org/0000-0003-4862-1300)
- Brittany Miller
- Aurore Demars (ORCID: https://orcid.org/0000-0002-7978-6880)
- Hugo L. P. Masson (ORCID: https://orcid.org/0009-0000-4156-8549)
- Renée M. Tsolis (ORCID: https://orcid.org/0000-0001-9131-6657)
- Andreas J. Bäumler (ORCID: https://orcid.org/0000-0001-9152-7809)
- Connor R. Tiffany (ORCID: https://orcid.org/0000-0001-8188-7720)
- Lauren C. Radlinski (ORCID: https://orcid.org/0000-0003-1863-5525)
- Megan J. Liou (ORCID: https://orcid.org/0000-0002-2489-5019)
- Anaïs Larabi
- Sophie R Gretler
- Lalita Bechtold
- Andrew W. Rogers (ORCID: https://orcid.org/0009-0003-0857-7939)
- Briana M. Young
- Henry Nguyen (ORCID: https://orcid.org/0000-0002-6737-1485)
Institutions
- University of California, Davis (US)
Publication Details
- Journal
- Cell Host & Microbe
- Published
- 2024-08-23
- DOI
- https://doi.org/10.1016/j.chom.2024.07.025
- Citations
- 36
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 8.42
Funders
- Kenneth Rainin Foundation
- National Institutes of Health
- U.S. Public Health Service
- University of California, Davis
- National Center for Advancing Translational Sciences
- Genome Center, University of California, Davis