Early and late gut microbiota signatures of stroke in high salt-fed stroke-prone spontaneously hypertensive rats
The high salt-fed stroke-prone spontaneously hypertensive rat (SHRSP) is a suitable tool to study the mechanisms underlying stroke pathogenesis. Salt intake modifies the gut microbiota (GM) in rats and humans and alterations of the GM have previously been associated with increased stroke occurrence. We aimed to characterize the GM profile in SHRSPs fed a high-salt stroke-permissive diet (Japanese diet, JD), compared to the closely related stroke-resistant control (SHRSR), to identify possible changes associated with stroke occurrence. SHRSPs and SHRSRs were fed a regular diet or JD for 4 weeks (short-term, ST) or a maximum of 10 weeks (long-term, LT). Stroke occurred in SHRSPs on JD-LT, preceded by proteinuria and diarrhoea. The GM of JD-fed SHRSPs underwent early and late compositional changes compared to SHRSRs. An overrepresentation of Streptococcaceae and an underrepresentation of Lachnospiraceae were observed in SHRSPs JD-ST, while in SHRSPs JD-LT short-chain fatty acid producers, e.g. Lachnobacterium and Faecalibacterium, decreased and pathobionts such as Coriobacteriaceae and Desulfovibrio increased. Occludin gene expression behaved differently in SHRSPs and SHRSRs. Calprotectin levels were unchanged. In conclusion, the altered GM in JD-fed SHRSPs may be detrimental to gut homeostasis and contribute to stroke occurrence.
Authors
- Augusta Zannoni (ORCID: https://orcid.org/0000-0002-2972-4921)
- Sara Roggiani (ORCID: https://orcid.org/0009-0008-2455-6624)
- Rosita Stanzione (ORCID: https://orcid.org/0000-0002-3576-4604)
- Maurizio Forte (ORCID: https://orcid.org/0000-0003-0739-0534)
- Gabriele Conti (ORCID: https://orcid.org/0000-0002-4623-043X)
- Marco Fabbrini (ORCID: https://orcid.org/0000-0003-1431-0643)
- Silvia Turroni (ORCID: https://orcid.org/0000-0003-2345-9482)
- Massimo Volpe (ORCID: https://orcid.org/0000-0002-9642-8380)
- Federica D’Amico (ORCID: https://orcid.org/0000-0002-2136-9558)
- Patrizia Brigidi (ORCID: https://orcid.org/0000-0001-8375-6150)
- Silvia Bencivenni (ORCID: https://orcid.org/0000-0002-2495-7320)
- Maria Cotugno (ORCID: https://orcid.org/0000-0003-0061-1154)
- Carla L. Busceti (ORCID: https://orcid.org/0000-0002-1417-1893)
- Francesco Fornai (ORCID: https://orcid.org/0000-0002-3883-5084)
- Monica Forni (ORCID: https://orcid.org/0000-0003-1310-6202)
- Donatella Pietrangelo
- Speranza Rubattu
- Margherita Litterio
Institutions
- University of Pisa (IT)
- Istituto Neurologico Mediterraneo (IT)
- IRCCS Ospedale San Raffaele (IT)
- Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele (IT)
- Sapienza University of Rome (IT)
- University of Bologna (IT)
Publication Details
- Journal
- Scientific Reports
- Published
- 2024-08-23
- DOI
- https://doi.org/10.1038/s41598-024-69961-9
- Citations
- 4
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- European Commission
- China University of Petroleum, Beijing
- Ministero della Salute