Inhibitory effects of extracts from <i>Eucalyptus gunnii</i> on α-synuclein amyloid fibrils
Amyloid fibril formation is associated with various amyloidoses, including neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Despite the numerous studies on the inhibition of amyloid formation, the prevention and treatment of a majority of amyloid-related disorders are still challenging. In this study, we investigated the effects of various plant extracts on amyloid formation of α-synuclein. We found that the extracts from Eucalyptus gunnii are able to inhibit amyloid formation, and to disaggregate preformed fibrils, in vitro. The extract itself did not lead to cell damage. In the extract, miquelianin, which is a glycosylated form of quercetin and has been detected in the plasma and the brain, was identified and assessed to have a moderate inhibitory activity, compared to the effects of ellagic acid and quercetin, which are strong inhibitors for amyloid formation. The properties of miquelianin provide insights into the mechanisms controlling the assembly of α-synuclein in the brain.
Authors
- Tomoyuki Oki (ORCID: https://orcid.org/0000-0003-4739-2737)
- Yasushi Kawata (ORCID: https://orcid.org/0000-0002-2744-1506)
- Masatomo So (ORCID: https://orcid.org/0000-0002-4815-4995)
- Hiroyuki Sasaki (ORCID: https://orcid.org/0000-0002-2067-6193)
- Yasuo Nagata (ORCID: https://orcid.org/0000-0001-8995-1109)
- Minako Kondo
- Kinichi Nakashima (ORCID: https://orcid.org/0000-0001-5901-9628)
- Hisaya Kawate
- Kaede Yamazaki
- Isao Suetake (ORCID: https://orcid.org/0000-0002-1246-8474)
- Hiroshi Hamajima
- Kuniyoshi Shimizu (ORCID: https://orcid.org/0000-0001-5960-1503)
- Charlotte Nachtegael (ORCID: https://orcid.org/0000-0002-5034-8975)
- Misaki Ono
- Shigeki Oogai (ORCID: https://orcid.org/0000-0002-4355-1465)
- Naoki Yamamoto (ORCID: https://orcid.org/0000-0003-2021-0104)
- Risa Mutoh (ORCID: https://orcid.org/0000-0003-0069-8931)
- Hironobu Hojo (ORCID: https://orcid.org/0000-0003-0916-1233)
- Motoko Unoki (ORCID: https://orcid.org/0000-0002-9629-3818)
- Akio Inoue (ORCID: https://orcid.org/0000-0002-0184-186X)
- Masaki Kato (ORCID: https://orcid.org/0000-0002-5184-1841)
- Kenichi Tanabe (ORCID: https://orcid.org/0000-0002-4386-535X)
- Toshiki Takei
- Noritaka Tokui
- S. Kumamoto
Institutions
- Université Libre de Bruxelles (BE)
- Vrije Universiteit Brussel (BE)
- Kyushu University (JP)
- Kyoto University (JP)
- Hamamatsu University School of Medicine (JP)
- Fukuoka University (JP)
- Saga Prefectural Education Center (JP)
- Institute of Bioinformatics (IN)
- Protein Research Foundation (JP)
- Tottori University (JP)
- Nakamura Gakuen University (JP)
- The University of Tokyo (JP)
- The University of Texas Southwestern Medical Center (US)
- Epigenomics (Germany) (DE)
- The University of Osaka (JP)
Publication Details
- Journal
- Bioscience Biotechnology and Biochemistry
- Published
- 2024-08-21
- DOI
- https://doi.org/10.1093/bbb/zbae114
- Citations
- 1
- Primary Topic
- Alzheimer's disease research and treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.33
Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Osaka University
- Institute for Protein Research, Osaka University