Single-nucleosome imaging unveils that condensins and nucleosome–nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes
For accurate mitotic cell division, replicated chromatin must be assembled into chromosomes and faithfully segregated into daughter cells. While protein factors like condensin play key roles in this process, it is unclear how chromosome assembly proceeds as molecular events of nucleosomes in living cells and how condensins act on nucleosomes to organize chromosomes. To approach these questions, we investigate nucleosome behavior during mitosis of living human cells using single-nucleosome tracking, combined with rapid-protein depletion technology and computational modeling. Our results show that local nucleosome motion becomes increasingly constrained during mitotic chromosome assembly, which is functionally distinct from condensed apoptotic chromatin. Condensins act as molecular crosslinkers, locally constraining nucleosomes to organize chromosomes. Additionally, nucleosome-nucleosome interactions via histone tails constrain and compact whole chromosomes. Our findings elucidate the physical nature of the chromosome assembly process during mitosis.
Authors
- Masa A. Shimazoe (ORCID: https://orcid.org/0000-0002-2018-0497)
- Masato T. Kanemaki (ORCID: https://orcid.org/0000-0002-7657-1649)
- Kazuhiro Maeshima (ORCID: https://orcid.org/0000-0003-3909-0341)
- Kayo Hibino (ORCID: https://orcid.org/0000-0002-4047-883X)
- Yuji Sakai (ORCID: https://orcid.org/0000-0002-2184-4304)
- Sachiko Tamura (ORCID: https://orcid.org/0000-0002-3121-6475)
- Katsuhiko Minami (ORCID: https://orcid.org/0000-0002-1750-5473)
- Naoko Imamoto (ORCID: https://orcid.org/0000-0002-2886-3022)
- Toyoaki Natsume (ORCID: https://orcid.org/0000-0002-3544-4491)
- Masatoshi Takagi (ORCID: https://orcid.org/0000-0003-4805-545X)
Institutions
- National Institute of Genetics (JP)
- The Graduate University for Advanced Studies, SOKENDAI (JP)
- Kyoto University (JP)
- RIKEN Center for Brain Science (JP)
- Tokyo Metropolitan Institute of Medical Science (JP)
- Pioneer (Japan) (JP)
- RIKEN (JP)
- Graduate School of Health Care Sciences, Jikei Institute (JP)
- The University of Tokyo (JP)
- Yokohama City University (JP)
Publication Details
- Journal
- Nature Communications
- Published
- 2024-08-21
- DOI
- https://doi.org/10.1038/s41467-024-51454-y
- Citations
- 24
- Primary Topic
- Genomics and Chromatin Dynamics
- Type
- article
- Field-Weighted Citation Impact
- 5.61
Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science
- RIKEN
- Core Research for Evolutional Science and Technology
- Precursory Research for Embryonic Science and Technology