Artificial Intelligence in Chromatin Analysis: A Random Forest Model Enhanced by Fractal and Wavelet Features

In this study, we propose an innovative concept that applies an AI-based approach using the random forest algorithm integrated with fractal and discrete wavelet transform features of nuclear chromatin. This strategy could be employed to identify subtle structural changes in cells that are in the early stages of programmed cell death. The code for the random forest model is developed using the Scikit-learn library in Python and includes hyperparameter tuning and cross-validation to optimize performance. The suggested input data for the model are chromatin fractal dimension, fractal lacunarity, and three wavelet coefficient energies obtained through high-pass and low-pass filtering. Additionally, the code contains several methods to assess the performance metrics of the model. This model holds potential as a starting point for designing simple yet advanced AI biosensors capable of detecting apoptotic cells that are not discernible through conventional microscopy techniques.