The human <scp>AMPKγ3 R225W</scp> mutation negatively impacts site‐1 nucleotide binding and does not enhance basal <scp>AMPKγ3</scp>‐associated activity nor glycogen production in human or mouse skeletal muscle
The AMPKγ3 R225W mutation does not impact the AMPK-associated activity in human skeletal muscle and the mutation is not linked to glycogen accumulation. The R225W mutation ablates the AMPKγ3-associated activation by AICAR/muscle contractions, presumably due to loss of nucleotide binding in the CBS 1 domain of AMPKγ3.
Authors
- Nicolas O. Eskesen (ORCID: https://orcid.org/0000-0002-3686-9264)
- Rasmus Kjøbsted (ORCID: https://orcid.org/0000-0002-0628-4994)
- Nicolai S. Henriksen (ORCID: https://orcid.org/0009-0008-7647-5535)
- Stine Ringholm (ORCID: https://orcid.org/0000-0001-8436-559X)
- Henriette Pilegaard (ORCID: https://orcid.org/0000-0002-1071-0327)
- Jørgen F. P. Wojtaszewski (ORCID: https://orcid.org/0000-0001-8185-3408)
- Nicoline R. Andersen (ORCID: https://orcid.org/0000-0002-1512-2727)
- Jesper B. Birk (ORCID: https://orcid.org/0000-0001-9775-4789)
Institutions
- University of Copenhagen (DK)
Publication Details
- Journal
- Acta Physiologica
- Published
- 2024-08-22
- DOI
- https://doi.org/10.1111/apha.14213
- Primary Topic
- Metabolism, Diabetes, and Cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00