Preliminary evaluation of the neuroprotective activity of Cannabidiol in combination with some current medicinal drugs <i>in vitro</i>

Objective:The objective of this study was to evaluate the neurotoxicity and neuroprotective activity of cannabidiol (CBD), valproate, furosemide, metformin, and bilobalide, individually and mixed with CBD in PC12 cells exposed to Glutamate.In addition, antioxidant activity, reactive oxygen species (ROS) production, and caspase-3 activity were evaluated.Methods: Neurotoxicity and neuroprotection were evaluated with the MTT assay; antioxidant activity, and production of ROS with 2,2-diphenyl-1-picryl-hydrazyl-hydrate, and DCFDA methods, respectively; the apoptosis with caspase-3 activity assay.Results: CBD 5 M, and bilobalide 15.3 M showed neuroprotective activity by ROS inhibition and decreased caspase-3 activity.Metformin 1548.4 M did not provide significant neuroprotection but decreased ROS generation.Conclusions: Combining CBD with the study drugs did not improve neuroprotection than the neuroprotection of individual drugs.