Physiological significance of the two isoforms of initiator tRNAs in <i>Escherichia coli</i>
<i>Escherichia coli</i> harbors four initiator tRNA (i-tRNA) genes: three of these at <i>metZWV</i> and the fourth one at <i>metY</i> loci. In <i>E. coli</i> B, all four genes encode i-tRNA<sup>fMet1</sup>. In <i>E. coli</i> K, because of a single-nucleotide polymorphism, metY encodes a variant, i-tRNA<sup>fMet2</sup>, having an A in place of G at position 46 of i-tRNA sequence in metY. We show that G46 confers stability to i-tRNA<sup>fMet1</sup>. The strains sustained on i-tRNA<sup>fMet1</sup> have a growth fitness advantage over those sustained on i-tRNA<sup>fMet2</sup>. Strains harboring <i>metY</i><sub>G46</sub> (B mimic) or <i>metY</i><sub>A46</sub> (K mimic) show that while in the nutrient-rich media, the K mimic is outcompeted rapidly; in the nutrient-poor medium, the K mimic is outcompeted less rapidly.
Authors
- Kuldeep Lahry (ORCID: https://orcid.org/0000-0002-6723-363X)
- Sunil Shetty (ORCID: https://orcid.org/0000-0002-8196-421X)
- Amit Kumar Sahu (ORCID: https://orcid.org/0000-0001-6740-9810)
- Riyaz Ahmad Shah (ORCID: https://orcid.org/0000-0002-7874-0928)
- Umesh Varshney (ORCID: https://orcid.org/0000-0003-3196-5908)
- Sudhir Singh (ORCID: https://orcid.org/0000-0001-9210-4605)
- Tanweer Hussain (ORCID: https://orcid.org/0000-0003-4735-2380)
- Prafful Sharma (ORCID: https://orcid.org/0000-0001-6562-7734)
- Rajagopal Varada (ORCID: https://orcid.org/0000-0001-6740-6564)
- Divya Nashier
Institutions
- Jawaharlal Nehru Centre for Advanced Scientific Research (IN)
- Indian Institute of Science Bangalore (IN)
Publication Details
- Journal
- Journal of Bacteriology
- Published
- 2024-08-22
- DOI
- https://doi.org/10.1128/jb.00251-24
- Primary Topic
- RNA and protein synthesis mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Department of Biotechnology, Ministry of Science and Technology, India
- Science and Engineering Research Board