Impact of carbon dioxide concentrations on laboratory sensitivity of <i>Mycoplasma</i> species isolated from dairy cows

Current <i>Mycoplasma</i> spp. culture protocols lack empirical derivation concerning carbon dioxide (CO<sub>2</sub>) supplementation and are primarily based on the initial cultivation of <i>Mycoplasma bovis</i>. This study indicates that the suitable range for CO<sub>2</sub> supplementation is broader than what is currently recommended by the National Mastitis Council for culturing within the specified 7-10 days. No differences in bacterial growth detection rates were observed among ambient air, 5% CO<sub>2</sub>, or 10% CO<sub>2</sub> supplementation during the 7- and 10-day incubation intervals. These new insights provide evidence supporting the possibility of culturing <i>Mycoplasma</i> spp. under ambient air conditions in a laboratory setting.

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Publication Details

Journal
Microbiology Spectrum
Published
2024-08-20
DOI
https://doi.org/10.1128/spectrum.00946-24
Primary Topic
Microbial infections and disease research
Type
article
Field-Weighted Citation Impact
0.00

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article

Impact of carbon dioxide concentrations on laboratory sensitivity of Mycoplasma species isolated from dairy cows

Paul J. Adams, Diego B. Nóbrega, G. Gioia, Erik van Engelen et al.
Microbiology Spectrum
Microbial infections and disease research
article

Impact of carbon dioxide concentrations on laboratory sensitivity of Mycoplasma species isolated from dairy cows

Paul J. Adams, Diego B. Nóbrega, G. Gioia, Erik van Engelen, Marit M. Biesheuvel, G. van Schaik, Jeroen De Buck, Herman W. Barkema, P. Moroni, Kristen N. Kalbfleisch, Jermaine R. Walcott, Gennerick J. Samera
article en

Abstract

Current <i>Mycoplasma</i> spp. culture protocols lack empirical derivation concerning carbon dioxide (CO<sub>2</sub>) supplementation and are primarily based on the initial cultivation of <i>Mycoplasma bovis</i>. This study indicates that the suitable range for CO<sub>2</sub> supplementation is broader than what is currently recommended by the National Mastitis Council for culturing within the specified 7-10 days. No differences in bacterial growth detection rates were observed among ambient air, 5% CO<sub>2</sub>, or 10% CO<sub>2</sub> supplementation during the 7- and 10-day incubation intervals. These new insights provide evidence supporting the possibility of culturing <i>Mycoplasma</i> spp. under ambient air conditions in a laboratory setting.

Microbiology SpectrumVol. 12(10)
University of Calgary (CA), University of Milan (IT), Utrecht University (NL), Cornell University (US), Kwantlen Polytechnic University (CA), Royal GD (Netherlands) (NL), New York State College of Veterinary Medicine (US)
Dairy Farmers of Canada, Dairy Farmers of Manitoba, Natural Sciences and Engineering Research Council of Canada
Openalex Percentile: Top 87%
Microbial infections and disease research
DOIOpen Access PDFOpenAlex
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