Phytochemical Analysis and <i>In Vivo</i> Antimalarial Activities of Ethyl Acetate Fraction of <i>Spilanthes filicaulis</i> on Mice Subjected to <i>Plasmodium berghei</i>

<b><i>Background and Objectives:</i></b> Malaria airs a life-threatening risk in Tropical African countries, stemming from infection by <i>Plasmodium</i> species. This region is richly endowed by nature with a wealth of diverse and largely unexplored plants that hold the potential for managing this protozoan parasite. The currently accessible over-the-counter drugs for disease management often present affordability challenges for the average person, exacerbated by the parasite's increasing resistance to them. This study investigated the phytoconstituents present in the ethyl acetate fraction of <i>Spilanthes filicaulis</i> (EFSF) and explored the antimalarial effects of EFSF on mice infected with <i>Plasmodium berghei</i>. <b><i>Methods:</i></b> Standard methods and gas chromatography-mass spectrometry (GC-MS) were used to identify phytoconstituents. Chloroquine phosphate-sensitive <i>P. berghei</i> (NK-65) was intraperitoneally inoculated into Swiss mice. The <i>in vivo</i> antimalarial activity of EFSF was assessed at dose levels of 250, 500, and 750 mg/kg, using 4-day suppressive and curative antimalarial models. Parameters evaluated in the inoculated mice included rectal temperature (RT), body weight (BW), packed cell volume (PCV), level of parasitemia, and mean survival time (MST). <b><i>Results:</i></b> Steroids, alkaloids, flavonoids, tannins, saponins, terpenoids, and cardiac glycosides were the identified phytochemicals present in EFSF, and GC-MS alongside reveals the presence of 20 bioactive compounds predominantly fatty acids and alcohol esters. Significant prevention of reductions in RT, BW, and PCV was observed in the EFSF-treated groups dose dependently relative to the untreated group. In addition, EFSF-treated groups significantly (<i>p</i> < 0.05) suppressed parasitemia and exhibited chemosuppression of 79.46% and 77.38% in 4-day suppressive, whereas suppression of 59.74% and 58.66% in curative treatment, respectively, at 500 and 750 mg/kg thus consequently extending the MST of infected treated mice compared with the untreated group. <b><i>Interpretation and Conclusion:</i></b> Put together, the EFSF exhibited enhanced antimalarial efficacy against mice infected with <i>P. berghei</i> thus affirming that plants still maintain lead way as a potential source of novel antimalarial remedies.