Detecting M-Protein via Mass Spectrometry and Affinity Beads: Enrichment With Mixed Kappa-Lambda Beads Enables Prompt Application in Clinical Laboratories
NBs for M-protein detection, particularly with mixed kappa-lambda beads, identified monoclonal peaks with both MALDI-TOF and ESI-qTOF analyses. Qualitative analysis using MALDI-TOF yielded results comparable with that of IFE. NB-MALDI-TOF might be used as an alternative method to replace conventional tests (such as IFE) to detect M-protein with high sensitivity.
Authors
- Heeyoun Hwang (ORCID: https://orcid.org/0000-0002-0124-2533)
- E. K. Kim
- Kyunghoon Lee (ORCID: https://orcid.org/0000-0002-3154-0347)
- Junghan Song (ORCID: https://orcid.org/0000-0003-0576-9938)
- Jikyo Lee (ORCID: https://orcid.org/0000-0002-7988-312X)
- Joon Hee Lee (ORCID: https://orcid.org/0000-0002-9649-1371)
- Sang Hoon Song (ORCID: https://orcid.org/0000-0002-5084-1137)
- Jung Hoon Choi (ORCID: https://orcid.org/0009-0004-6402-8915)
- Seungman Park (ORCID: https://orcid.org/0000-0003-2123-3711)
- Jihyun Im
- Seojin Yang (ORCID: https://orcid.org/0009-0003-0146-1095)
Institutions
- Seoul National University (KR)
- Chungnam National University (KR)
- Seoul National University Hospital (KR)
- Seoul National University Bundang Hospital (KR)
- National Cancer Center (KR)
- Korea Basic Science Institute (KR)
Publication Details
- Journal
- Annals of Laboratory Medicine
- Published
- 2024-08-20
- DOI
- https://doi.org/10.3343/alm.2024.0039
- Citations
- 2
- Primary Topic
- Multiple Myeloma Research and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.91
Funders
- National Research Council of Science and Technology
- Ministry of Science and ICT, South Korea