Effect of BA on Callus Induction, Shoot and Root Development from Callus of Nepenthes ampullaria through In Vitro Culture

Nepenthes ampullaria Jack. or pitcher plant is a carnivorous plant in the Nepenthaceae family. They are in danger of extinction because of the less forest area, volatile weather, and pollution. This study aimed to investigate the influence of BA (6-benzyl adenine) on callus induction of Nepenthes sp. through In vitro culture. The experiment was divided into three parts; (1) Callus induction; shoot of a pitcher plant were cultured on solid MS medium (Murashige & Skoog, 1962) supplemented with BA (6-benzyladenine) at 0, 1, 2, and 4 mg/l for 70 days. The experiment design was completely Randomized Design (CRD) (4 treatments X 10 replicates). It was found that MS medium supplemented with 4 mg/l BA proved to be a suitable medium for callus induction (70%). (2) Shoot induction from callus; callus were cultured on MS medium supplemented with 0, 2, and 4 mg/l BA (CRD, 3 treatments X 10 replicates) for 45 days. The highest average number of regenerated shoots (19.7±0.3 shoots) was observed on MS medium without BA. (3) Rooting induction; the young shoots, 1-2 cm in length, were cultured on MS medium containing 0, 1, 3, and 5 mg/l NAA combination with 0 and 3 mg/l IBA for 40 days (CRD, 8 treatments X 10 replicates). The results showed that the MS medium supplemented with 1 mg/l NAA and 3 mg/l IBA was suitable for root induction with the average of 7.4±1.6 roots.