Fabrication of tetra‐n‐butylammonium hydrogen sulfate‐based poly(lactic acid)‐poly(ethylene glycol) composite electrospun wound dressing

Abstract In this study, poly(lactic acid)‐poly(ethylene glycol)‐tetra‐n‐butylammonium hydrogen sulfate (PLA‐PEG‐HS) electrospun mats were fabricated by electrospinning as effective wound dressings against Staphylococcus aureus ( S . aureus ) and Escherichia coli ( E . coli ), which are the most prevalent species of gram‐positive and gram‐negative bacteria, respectively. PLA is a polymer with high biocompatibility and biodegradability, but its structure is fragile. Therefore, we aimed to improve its structural properties using PEG as a plasticizer and to provide antibacterial properties with HS salt. The nanofibers characterized using differential scanning calorimetry (DSC), Fourier transform infrared (FTIR) spectroscopy, thermal gravimetric analysis (TGA), and drying time tests. The addition of HS eliminated the beads in the PLA‐PEG nanofiber and improved the homogeneity of the fiber distribution. Concerning this result, when the liquid absorption capacity (LAC) test was evaluated, PLA‐PEG‐HS nanofiber production was achieved with the highest rate of 480.95%. The thermal properties of nanofibers increased with the addition of HS. Cytotoxicity test results of PLA‐PEG‐HS wound dressings showed high cell viability of 129.05% in L292 mouse fibroblast cells at the end of the 24th hour. PLA‐PEG‐HS nanofibers with 99.99% antibacterial activity against tow bacteria. Considering the modern wound dressing requirements, antibacterial wound dressing production has been successfully achieved.