CB2 expression in mouse brain: from mapping to regulation in microglia under inflammatory conditions
Since its detection in the brain, the cannabinoid receptor type 2 (CB2) has been considered a promising therapeutic target for various neurological and psychiatric disorders. However, precise brain mapping of its expression is still lacking. Using magnetic cell sorting, calibrated RT-qPCR and single-nucleus RNAseq, we show that CB2 is expressed at a low level in all brain regions studied, mainly by few microglial cells, and by neurons in an even lower proportion. Upon lipopolysaccharide stimulation, modeling neuroinflammation in non-sterile conditions, we demonstrate that the inflammatory response is associated with a transient reduction in CB2 mRNA levels in brain tissue, particularly in microglial cells. This result, confirmed in the BV2 microglial cell line, contrasts with the positive correlation observed between CB2 mRNA levels and the inflammatory response upon stimulation by interferon-gamma, modeling neuroinflammation in sterile condition. Discrete brain CB2 expression might thus be up- or down-regulated depending on the inflammatory context.
Authors
- Cyril Dégletagne (ORCID: https://orcid.org/0000-0002-5363-2381)
- Sylvain Rheims (ORCID: https://orcid.org/0000-0002-4663-8515)
- Anne Ruiz (ORCID: https://orcid.org/0000-0002-6741-0958)
- Guillaume Marcy (ORCID: https://orcid.org/0000-0002-3469-8131)
- Nadia Gasmi (ORCID: https://orcid.org/0000-0002-3694-333X)
- Jacques Bodennec (ORCID: https://orcid.org/0009-0004-6482-4866)
- Laurent Bezin (ORCID: https://orcid.org/0000-0001-6411-4267)
- Amor Belmeguenaï (ORCID: https://orcid.org/0000-0003-3295-3095)
- Wanda Grabon (ORCID: https://orcid.org/0000-0003-4443-476X)
- Béatrice Georges
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Centre de Recherche en Neurosciences de Lyon (FR)
- Centre Léon Bérard (FR)
- Centre de Recherche en Cancérologie de Lyon (FR)
Publication Details
- Journal
- Journal of Neuroinflammation
- Published
- 2024-08-19
- DOI
- https://doi.org/10.1186/s12974-024-03202-8
- Citations
- 23
- Primary Topic
- Cannabis and Cannabinoid Research
- Type
- article
- Field-Weighted Citation Impact
- 10.30
Funders
- Institut National de la Santé et de la Recherche Médicale
- Fondation pour la Recherche Médicale
- Association France Alzheimer
- Centre National de la Recherche Scientifique
- Université Claude Bernard Lyon 1