Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study

As our study, as well as most of the previous studies, has shown RT-ddPCR to be more sensitive than RT-qPCR, its use in the wastewater surveillance of SARS-CoV-2 should be considered, especially if the amount of SARS-CoV-2 circulating in the population was low. All the analysis steps must be optimized for wastewater surveillance as our study showed that all the analysis steps including the compatibility of the RNA extraction, the RT-PCR kit, and the target gene assay influence the results. In addition, our study showed that RT-SIBA could be used to detect SARS-CoV-2 in wastewater if a qualitative result is sufficient.

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Publication Details

Journal
JMIR Public Health and Surveillance
Published
2024-08-19
DOI
https://doi.org/10.2196/53175
Citations
4
Primary Topic
SARS-CoV-2 detection and testing
Type
article
Field-Weighted Citation Impact
1.56
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article

Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study

Tarja Pitkänen, Annamari Heikinheimo, Anssi Lipponen, Sami Oikarinen et al.
4 citations
JMIR Public Health and Surveillance
SARS-CoV-2 detection and testing
1.56
article

Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study

Tarja Pitkänen, Annamari Heikinheimo, Anssi Lipponen, Sami Oikarinen, K. Lehto, Rafiqul Hyder, Annika Länsivaara, Erja Janhonen
article en
4 citations

Abstract

As our study, as well as most of the previous studies, has shown RT-ddPCR to be more sensitive than RT-qPCR, its use in the wastewater surveillance of SARS-CoV-2 should be considered, especially if the amount of SARS-CoV-2 circulating in the population was low. All the analysis steps must be optimized for wastewater surveillance as our study showed that all the analysis steps including the compatibility of the RNA extraction, the RT-PCR kit, and the target gene assay influence the results. In addition, our study showed that RT-SIBA could be used to detect SARS-CoV-2 in wastewater if a qualitative result is sufficient.

JMIR Public Health and SurveillanceVol. 10
University of Helsinki (FI), Tampere University (FI), Finnish Institute for Health and Welfare (FI), Finnish Food Authority (FI)
Clean water and sanitation
Openalex Percentile: Top 17%
SARS-CoV-2 detection and testing
1.56
DOIOpen Access PDFOpenAlex
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Comparison of Different Reverse Transcriptase–Polymerase Chain Reaction–Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study — Tarja Pitkänen, Annamari Heikinheimo, et al. · JMIR Public Health and Surveillance (2024) | TGRS Research Map | TGRS