DNA Vaccines Encoding HTNV GP-Derived Th Epitopes Benefited from a LAMP-Targeting Strategy and Established Cellular Immunoprotection
Vaccines has long been the focus of antiviral immunotherapy research. Viral epitopes are thought to be useful biomarkers for immunotherapy (both antibody-based and cellular). In this study, we designed a novel vaccine molecule, the Hantaan virus (HTNV) glycoprotein (GP) tandem Th epitope molecule (named the Gnc molecule), in silico. Subsequently, computer analysis was used to conduct a comprehensive and in-depth study of the various properties of the molecule and its effects as a vaccine molecule in the body. The Gnc molecule was designed for DNA vaccines and optimized with a lysosomal-targeting membrane protein (LAMP) strategy. The effects of GP-derived Th epitopes and multiepitope vaccines were initially verified in animals. Our research has resulted in the design of two vaccines based on effective antiviral immune targets. The effectiveness of molecular therapies has also been preliminarily demonstrated in silico and in laboratory animals, which lays a foundation for the application of a vaccines strategy in the field of antivirals.
Authors
- Yongkai Wang (ORCID: https://orcid.org/0000-0002-6399-6955)
- Baozeng Sun (ORCID: https://orcid.org/0009-0005-3892-7359)
- Ruibo Liu (ORCID: https://orcid.org/0000-0002-5163-966X)
- Shuya Yang (ORCID: https://orcid.org/0000-0001-8884-7254)
- Jijin Li
- Dongbo Jiang (ORCID: https://orcid.org/0000-0002-2675-5634)
- Chenjin Ma (ORCID: https://orcid.org/0000-0002-2483-2309)
- Zhikui Li
- Jiawei Wang (ORCID: https://orcid.org/0000-0001-5009-5640)
- Jiaxing Zhang (ORCID: https://orcid.org/0000-0002-0178-7007)
- Jinpeng Zhang (ORCID: https://orcid.org/0000-0003-0995-935X)
- Yueyue Wang (ORCID: https://orcid.org/0000-0002-2178-3927)
- Linfeng Cheng
- Yubo Sun (ORCID: https://orcid.org/0000-0003-2998-6574)
- Zhen-Jie Wang (ORCID: https://orcid.org/0000-0002-6752-1690)
- Tianyuan Bai
- Zilu Ma
- Kun Yang (ORCID: https://orcid.org/0000-0003-0305-6256)
- Guanwen Zhang (ORCID: https://orcid.org/0009-0003-0413-5412)
- Yuanjie Sun (ORCID: https://orcid.org/0009-0009-6682-1817)
- Junqi Zhang (ORCID: https://orcid.org/0009-0001-4733-023X)
- Wenyang Shen
- Sirui Cai
- Gefei Zhang
Institutions
- Chinese People's Armed Police Force
- Air Force Medical University (CN)
Publication Details
- Journal
- Vaccines
- Published
- 2024-08-19
- DOI
- https://doi.org/10.3390/vaccines12080928
- Citations
- 1
- Primary Topic
- Viral Infections and Vectors
- Type
- article
- Field-Weighted Citation Impact
- 0.39
Funders
- National Natural Science Foundation of China
- Yakult Bio-Science Foundation
- Key Research and Development Projects of Shaanxi Province