Improving normothermic machine perfusion and blood transfusion through biocompatible blood silicification

The growing world population and increasing life expectancy are driving the need to improve the quality of blood transfusion, organ transplantation, and preservation. Here, to improve the ability of red blood cells (RBCs) for normothermic machine perfusion, a biocompatible blood silicification approach termed "shielding-augmenting RBC-in-nanoscale amorphous silica (SARNAS)" has been developed. The key to RBC surface engineering and structure augmentation is the precise control of the hydrolysis form of silicic acid to realize stabilization of RBC within conformal nanoscale silica-based exoskeletons. The formed silicified RBCs (Si-RBCs) maintain membrane/structural integrity, normal cellular functions (e.g., metabolism, oxygen-carrying capability), and enhance resistance to external stressors as well as tunable mechanical properties, resulting in nearly 100% RBC cryoprotection. In vivo experiments confirm their excellent biocompatibility. By shielding RBC surface antigens, the Si-RBCs provide universal blood compatibility, the ability for allogeneic mechanical perfusion, and more importantly, the possibility for cross-species transfusion. Being simple, reliable, and easily scalable, the SARNAS strategy holds great promise to revolutionize the use of engineered blood for future clinical applications.

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Publication Details

Journal
Proceedings of the National Academy of Sciences
Published
2024-08-19
DOI
https://doi.org/10.1073/pnas.2322418121
Citations
14
Primary Topic
Medical Imaging and Pathology Studies
Type
article
Field-Weighted Citation Impact
5.87

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article

Improving normothermic machine perfusion and blood transfusion through biocompatible blood silicification

Liang Zhou, C. Jeffrey Brinker, Qi Lei, Romy Ettlinger et al.
14 citations
Proceedings of the National Academy of Sciences
Medical Imaging and Pathology Studies
5.87
article

Improving normothermic machine perfusion and blood transfusion through biocompatible blood silicification

Liang Zhou, C. Jeffrey Brinker, Qi Lei, Romy Ettlinger, Junxian Yang, Xuejin Li, Qing Ouyang, Jiangguo Lin, Jimin Guo, Shuhao Ma, Stefan Wuttke, Chuanyi Lei, Qi Zhang, Zeyu Li, Wei Zhu
article en
14 citations

Abstract

The growing world population and increasing life expectancy are driving the need to improve the quality of blood transfusion, organ transplantation, and preservation. Here, to improve the ability of red blood cells (RBCs) for normothermic machine perfusion, a biocompatible blood silicification approach termed "shielding-augmenting RBC-in-nanoscale amorphous silica (SARNAS)" has been developed. The key to RBC surface engineering and structure augmentation is the precise control of the hydrolysis form of silicic acid to realize stabilization of RBC within conformal nanoscale silica-based exoskeletons. The formed silicified RBCs (Si-RBCs) maintain membrane/structural integrity, normal cellular functions (e.g., metabolism, oxygen-carrying capability), and enhance resistance to external stressors as well as tunable mechanical properties, resulting in nearly 100% RBC cryoprotection. In vivo experiments confirm their excellent biocompatibility. By shielding RBC surface antigens, the Si-RBCs provide universal blood compatibility, the ability for allogeneic mechanical perfusion, and more importantly, the possibility for cross-species transfusion. Being simple, reliable, and easily scalable, the SARNAS strategy holds great promise to revolutionize the use of engineered blood for future clinical applications.

Proceedings of the National Academy of SciencesVol. 121(35)
Ikerbasque (ES), University of St Andrews (GB), University of New Mexico (US), Anhui Medical University (CN), Basque Center for Materials, Applications and Nanostructures (ES), Third Affiliated Hospital of Southern Medical University (CN), Guangdong Academy of Medical Sciences (CN), Second Affiliated Hospital of Anhui Medical University (CN), Guangdong Provincial People's Hospital (CN), Zhejiang University (CN), South China University of Technology (CN)
National Natural Science Foundation of China, Natural Science Foundation of Guangdong Province, Guangdong Provincial Pearl River Talents Program, Fundamental Research Funds for the Central Universities, Basic and Applied Basic Research Foundation of Guangdong Province
Responsible consumption and production
Openalex Percentile: Top 3%
Medical Imaging and Pathology Studies
5.87
DOIOpen Access PDFOpenAlex
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