Effects of using different biological products on IL-1β TNF-α, PGE2 concentrations in rabbits with periodontitis

Introduction: Platelet‐rich plasma (PRP) is a plasma fraction of autologous blood with a high platelet concentration, containing key cytokines and growth factors.Methods: This study, conducted in the Clinical Surgery Department of the College of Dentistry, University of Al-Muthanna, involved 20 New Zealand white rabbits divided into four groups. A 1mm gingival wound was created on each rabbit, followed by immediate injections:10μl saline10μl PRP10μl Platelet‐poor plasma (PPP)3ng hepatocyte growth factor (HGF) in 10μl engineered tendon matrixRabbits were sacrificed on days 0, 1, 3, 5, and 12 for tissue harvesting.Results: Interleukin 1 beta Increased in PPP group compared to HGF, PRP, and control groups. Tumor Necrosis Factor alpha: Increased in PPP group.While,PRP significantly reduced PGE2 levels on days 1, 3, and 5. On day 12, levels were low in both PRP and control groups. PPP slightly reduced PGE2 on days 1, 3, and 5. HGF reduced PGE2 on days 1, 3, and 12, with a transient response on day 0.Conclusion: PRP sustains the up-regulation of proinflammatory factors like Interleukin 1 beta and Tumor Necrosis Factor alpha. Its anti-inflammatory function is partially mediated through HGF, producing similar anti-inflammatory effects.