A comparative analysis of beta-cypermethrin nanoemulsion and traditional emulsion dosages on enzyme activities in Blattella germanica

To compare the impact of two pesticide dose forms on <i>Blattella germanica</i> (<i>B. germanica</i>)resistance enzymes.The micro-drop technique was utilized on subcultured <i>B. germanica</i>, and the metabolic enzyme activity was assessed.Using spectrophotometry, the relative enzyme activities of <i>B.germanica</i> were determined.Profile analysis was used to compare the enzyme activities of beta-cypermethrin in both nanoemulsion and traditional emulsion forms.Findings: The suppression percentages of the acetylcholinesterase enzyme (AchE), GST, and P450-O demethylases across different pesticide formulations were analyzed using regression equations, yielding <i>F</i> = 31.18, P < 0.001; <i>F</i> = 9.18, P < 0.001; and <i>F</i> = 4.58, P < 0.02.Conclusion: The suppression of AchE, GST, and P450-O demethylase by beta-cypermethrin nanoemulsion was more significant compared to the beta-cypermethrin emulsifiable concentrate.The study concluded that beta-cypermethrin nanoemulsion had a significant impact on insect detoxifying enzymes compared to beta-cypermethrin emulsifiable concentrate due to their numerous advantages.